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KMID : 0363320050260010074
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Journal of Korean Oriental Internal Medicine
2005 Volume.26 No. 1 p.74 ~ p.92
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The Effects of Injinchunggantang on Interferon Signaling Pathway of HepG2 Cells
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Lee Jong-Hoon
Kim Young-Chul
Lee Jang-Hoon
Woo Hong-Jung
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Abstract
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Objectives/Methods : To analyze the effect of Injinchunggantang(IJCGT) to Interferon-{alpha}/{beta} signal transmission system in HepG2 cells, HepG2 Cell were treated with IJCGT. Also, revelation of MxA, 2’5’-OAS mRNA leaded by Interferon-{alpha}/{beta} and revelation and activation of Jak1, TYK1, and STAT 1, all main signal transmission factors, were analyzed.
Results : The analysis resulted in the following
1. With interferon {alpha}/{beta} there was no affect cell propagation of Hep G2 cells. With IJCGT alone, cell propagation of HepG2 was promoted, and cell propagation control function was recovered.
2. With interferon {alpha}/{beta} cell death was unaffected. With IJCGT apoptosis of HepG2 cell was restrained, and the cell’s reaction to interferon was unaffected.
3. With interferon {alpha}/{beta} treatment mRNA revelation of MxA and 2’5’-OAS was induced. When HepG2 cells were injected with IJCGT without interferon {alpha}/{beta} treatment, mRNA revelation of MxA and 2’5’-OAS increased in proportion to the treatment density. With pre-treatment of IJCGT, leaded with interferon {alpha}/{beta}, promoted revelation of MxA, 2’5’ -OAS mRNA.
4. Though mRNA revelation of lakl, TYK1 and STAT1 was unaffected with IJCGT, activation of STAT1 was promoted with an increase of phosphorylation of STAT1 protein. With pre-treatment of IJCGT, Jak1, TYK2, STAT1 phosphorylation, leaded with interferon, strengthened.
5. TNF-a, IL-1b and LPS present, revelation of MxA and 2’5’-OAS mRNA leaded by interferon was restrained when HepG2 cells were treated with IJCGT, and the interferon signal transmission system restraint action leaded by inflammatory cytokines was moderated.
Conclusion : These results support a role for IJGCT in promotion of anti-virus action through maintainance of the liver’s sensibility toward interferon. A clinical study of an interferon treated patient treated also with IJGCT is needed to determine its efficacy.
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KEYWORD
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Interferon-{alpha}/{beta}, Injinchunggantang(IJCGT), Jak-STAT, MxA, 2’5’-OAS
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